Anti-PCNA Antibody, Rabbit Polyclonal
產(chǎn)品編號：CP00140HuA10
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產(chǎn)品名稱：Anti-PCNA Antibody, Rabbit Polyclonal
經(jīng)驗(yàn)證的應(yīng)用：WB/ICC
交叉反應(yīng)：/
特異性：human PCNA
免疫原：Recombinant human PCNA protein, fragment Met1~Ser261; UniprotKB: P12004
制備方法：Produced in rabbits immunized with human PCNA, and purified by antigen affinity chromatography.
來源：Polyclonal Rabbit IgG
純化：Immunogen affinity purified
緩沖液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶聯(lián)物：Unconjugated
狀態(tài)：Liquid
運(yùn)輸方式：This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
儲存條件：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
圖片：
Figure1.Western blot analysis of extracts of various cell lines using PCNA antibody (CP00140HuA10) at 1:1000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 10% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5s
Figure2.Western blot analysis of extracts of various cell lines using PCNA antibody (CP00140HuA10) at 1:1000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 10% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 3s
Figure3.Immunocytochemistry analysis of Huh7 (Human Hepatoma cells) cells labeling PCNA with purified CP00140HuA10 at 5 µg/mL. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100.Cells were counterstained with CY3 conjugated Anti-mouse IgG(H+L) (GB21301) 1/200 (2.5 µg/mL). Goat anti-mouse IgG (Alexa Fluor® 488, GB25301) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Figure4.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Canine breast cancer tissue sections labelling PCNA with purified PA00140HuA10 at 5 ug/ml . Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control.
背景信息：Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerases processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3-5 exonuclease and 3-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. This antibody is a rabbit polyclonal antibody raised against an internal region of human PCNA. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa （PMID: 1358458）.
全稱：Proliferating Cell Nuclear Antigen (PCNA)